کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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70127 | 48812 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation](/preview/png/70127.png)
Feruloyl esterases (FAEs) are a group of important industrial enzymes, which could hydrolyze the ester bonds between hydroxycinnamic acids and arabinose or galactose in hemicellulose present in plant cell walls. To establish a practical high-throughput screening system of feruoyl esterases, two new substrates with modified chromo- or fluoro-phore, 2-chloro-4-nitrophenyl ferulate (CNPF; 1b) and umbelliferyl 5-O-feruolyl-α-L-arabinofuranoside (UFA; 2a), were designed and synthesized. Both substrates provided significantly improved signal-to-noise ratio compared with previously known structural analogues. The chromogenic substrate CNPF could be readily adapted to the high-throughput screening of feruloyl esterase A from Aspergillus niger, one of the most studied FAEs, with the coefficient of variance of as low as 10.3% as determined for a single sequence library.
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► Two new mimic substrates with modified chlorophore or fluorophore were synthesized.
► Each new substrate provides significantly improved signal-to-noise ratio.
► A high-throughput screening system was developed for feruloyl esterases.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 74, Issues 1–2, January 2012, Pages 36–40