کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
70568 48838 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heterologous expression of an extra-cellular lipase from the basidiomycete Pleurotus sapidus
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Heterologous expression of an extra-cellular lipase from the basidiomycete Pleurotus sapidus
چکیده انگلیسی

The 1641 bp cDNA encoding an extra-cellular lipase of the basidiomycete Pleurotus sapidus (Lip2) was cloned from a cDNA library. Expression of the cDNA in Escherichia coli, with and without signal sequence, led to the production of recombinant Lip2, mainly as inclusion bodies with low catalytic activity. Refolding yielded catalytically active protein. A C-terminal His tag was used for purification and immunochemical detection. The recombinant lipase hydrolysed xanthophyll esters with high efficiency, and omitting the signal sequence did not alter the catalytic properties. The P. sapidus lipase represents the first enzyme of the lipase/esterase family from a basidiomycetous fungus characterised on the molecular level and expressed in a manageable host.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 57, Issues 1–4, May 2009, Pages 16–21
نویسندگان
, , , , ,