کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
70612 | 48838 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular modeling of substrate selectivity of Candida antarctica lipase B and Candida rugosa lipase towards c9, t11- and t10, c12-conjugated linoleic acid
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Molecular modeling was used to clarify the mechanism of the selectivity of Candida antarctica lipase B and Candida rugosa lipase towards cis9, trans11 (c9, t11-) and trans10, cis12 (t10, c12-) conjugated linoleic acid. Hydrogen bonds network, substrate conformation, binding affinity and water molecules in the binding site were analyzed. Substrate conformation and binding affinity were not correlated with the experimental results of the substrate selectivity. On the contrary, better enzyme preference towards a substrate was correlated with two stronger hydrogen bonds (His-NɛH-Oa and His-NɛH-Ser-Oγ) and less water molecules between the substrate the binding pocket. Possible explanation of these was discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 57, Issues 1–4, May 2009, Pages 299–303
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 57, Issues 1–4, May 2009, Pages 299–303
نویسندگان
Wencheng Li, Bo Yang, Yonghua Wang, Dongqing Wei, Chris Whiteley, Xiaoning Wang,