کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
70722 | 48843 | 2009 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Resolution of biotin intermediate lactone by enzyme-catalyzed stereoselective lactonization in organic solvent Resolution of biotin intermediate lactone by enzyme-catalyzed stereoselective lactonization in organic solvent](/preview/png/70722.png)
A strain of Aspergillus oryzae WZ007 producing intracellular lipase with preference for the formation of the d-biotin intermediate lactone ((3aS,6aR)-tetrahydro-1,3-dibenzylhexahydro-1H-Furo[3,4-d]imidazole-2,4-dione) 1 was screened from soil samples. The microbial cells fermented by the strain were used as biocatalysts after lyophilization, to resolve the racemic biotin intermediate lactone 1 by enantioselective lactonization in organic solvents. The enantioselective lactonization afforded optically active lactone 1 with high enantiomeric excess (e.e. ≥ 98%) and conversion ratio (c ≥ 40%) when the biotransformation was carried out in dichloromethane. The e.e. of product was up to 99% after purification by inducing crystallization in acetone and basically satisfied the industrial application. The biosynthesis has been employed to produce kilograms supplies of optically active lactone 1 with high optical purity.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 56, Issue 1, January 2009, Pages 20–23