کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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70740 | 48844 | 2011 | 6 صفحه PDF | دانلود رایگان |
Deoxyriboaldolase from Klebsiella pneumonia (KpDERA) was immobilized on interparticle pore type mesoporous silica (IMS), which consists of aggregates of hexagonally close-packed silica nanoparticles. Incubation of the free KpDERA in 300 mM acetaldehyde resulted in a decrease in the free enzyme activity to 20% of the initial activity after incubation for 30 min. No activity was observed after 90 min incubation. In contrast, the IMS-immobilized KpDERA retained more than 50% of its activity after 30 min and 11% activity after 90 min incubation. The product yield of a sequential aldol condensation reaction, which is central to the production of pharmaceutically important intermediates, was found to be higher for IMS-immobilized KpDERA than for the free enzyme. These results suggest the potential utility of IMS as an immobilization support for DERA.
Figure optionsDownload as PowerPoint slideResearch highlights▶ KpDERA was successfully immobilized on interparticle pore type mesoporous silica. ▶ IMS immobilized KpDERA exhibited superior aldehyde tolerance. ▶ Higher amount of the aldol condensation products was obtained with IMS-KpDERA.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 68, Issue 2, February 2011, Pages 181–186