کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7078937 | 1459987 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Divergent responses of functional gene expression to various nutrient conditions during microcystin-LR biodegradation by Novosphingobium sp. THN1 strain
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کلمات کلیدی
MCLRTCCMicrocystinsqPCRDADMCsQuantitative PCR - PCR کمیdiode array detector - آشکارساز آرایه دیودGene expression - بیان ژنALS - بیماری اسکلروز جانبی آمیوتروفیکBiodegradation - تجزیه زیستیmicrocystin-LR - میکروسیستین LRSodium nitrate - نیترات سدیمpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراAmmonium chloride - کلرید آمونیوم
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To better understand the mechanisms for microcystin-LR (MCLR) biodegradation, the linkage between MCLR-biodegradation kinetics and functional gene expression dynamics was originally investigated with Novosphingobium sp. THN1 as inoculum under various nutrient conditions. Along biodegradation, mlrA gene expression, coupled with mlrD, presented similar trend but was regulated differentially among different conditions. Good positive correlation was observed between MCLR degraded and induction ratios of functional genes until 42Â h at respective condition. Compared to those under nutrient-free condition, the stimulated or decelerated biodegradation with dipotassium phosphate (DP) or ammonium chloride (AC) (both at 100Â mg Lâ1) was related to higher or lower up-regulation in mlr gene expression, suggesting that divergent mlr gene expression was one of the reasons for different effects of DP or AC on degradation. However, stimulated degradation with sodium nitrate (100Â mg Lâ1) might involve other mechanisms where mlr expression was not the decisive prerequisite to govern MCLR-biodegradation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 156, March 2014, Pages 335-341
Journal: Bioresource Technology - Volume 156, March 2014, Pages 335-341
نویسندگان
Jieming Li, Liujing Peng, Ji Li, Yuhui Qiao,