کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7081531 | 1460000 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The role of carbohydrate binding module (CBM) at high substrate consistency: Comparison of Trichoderma reesei and Thermoascus aurantiacus Cel7A (CBHI) and Cel5A (EGII)
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کلمات کلیدی
CBMPWSCBHHPAEC-PADEndoglucanase - اندوگلیکونازCellulases - سلولزcellobiohydrolase - سلولی بیو هیدرولازdry matter - ماده خشکcellulose binding module - ماژول اتصال سلولزcarbohydrate binding module - ماژول اتصال کربوهیدراتLignocellulose - مواد لیگنوسلولزیEnzymatic hydrolysis - هیدرولیز آنزیمیpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول high performance anion exchange chromatography with pulsed amperometric detection - کروماتوگرافی مبدل آنیون با عملکرد بالا با تشخیص آمپومتریک پالسglycoside hydrolase - گلیکوزید هیدرولاز
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The role of CBM in two fungal model cellulase systems, consisting of Cel7A and Cel5A, from Trichoderma reesei and Thermoascus aurantiacus, were compared in the hydrolysis of various substrates. For comparison, family-1 CBM's were introduced to the T. aurantiacus and removed from the T. reesei enzymes. Especially at high dry matter consistencies of lignocellulosic substrates, pretreated wheat straw and spruce, the T. aurantiacus enzymes lacking CBM outperformed the enzymes carrying the CBM. In these conditions, the CBM-less enzymes from both organisms obviously recognized and bound to the substrate at higher probability than in dilute systems. Avoiding the unproductive binding to lignin caused by the CBMs obviously enhanced the hydrolytic performance. The lignin binding effect was, however, not entirely caused by the CBM, but also by the different structures and affinities of the core enzymes to lignin. Due to decreased binding, the CBM-less enzymes would allow reuse, potentially decreasing hydrolysis costs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 143, September 2013, Pages 196-203
Journal: Bioresource Technology - Volume 143, September 2013, Pages 196-203
نویسندگان
Tinaïg Le Costaouëc, Annukka Pakarinen, Anikó Várnai, Terhi Puranen, Liisa Viikari,