کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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70871 | 48850 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Hydroxylation of steroid compounds by Gelasinospora retispora Hydroxylation of steroid compounds by Gelasinospora retispora](/preview/png/70871.png)
Biotransformation of androst-4-ene-3,17-dione (1), 3β-hydroxypregnan-5-en-20-one (pregnenolone, 2), 3β-hydroxyandrost-5-en-17-one (DHEA, 3) and estradiol (4) was investigated with fungus of Gelasinospora retispora. Biotransformation of 1 gave 11α-hydroxyandrost-4-ene-3,17-dione (5) in good yield. In the case of compound 2, three compounds, DHEA (3), 3β,17β-dihydroxyandrost-5-ene (6) and 3β,15β-dihydroxyandrost-5-en-17-one (7) were obtained. Moreover, DHEA (3) was converted to 3β,7α-dihydroxyandrost-5-en-17-one (8) and 3β,11α-dihydroxyandrost-5-ene-7,17-dione (9). And it was found that biotransformation of 4 affords 6β-hydroxyestradiol (10).
Figure optionsDownload as PowerPoint slideResearch highlights▶ Biotransformation of androst-4-ene-3,17-dione using Gelasinospora retispora gave 11α-hydroxyandrost-4-ene-3,17-dione with good yield. ▶ Transformation of pregnenolone afforded three products: DHEA, 3β,17α-dihydroxyandrost-5-ene and 3β,15β-dihydroxyandrost-5-en-17-one. ▶ The side-chain of pregnenolone was cleaved and then hydroxylated at C-15β using G. retispora.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 67, Issues 1–2, November 2010, Pages 72–77