کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
71174 | 48880 | 2007 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Preparation and characterization of combi-CLEAs catalyzing multiple non-cascade reactions Preparation and characterization of combi-CLEAs catalyzing multiple non-cascade reactions](/preview/png/71174.png)
A novel cross-linked enzyme aggregates (CLEA) concept called combi-CLEA has been described. It is based upon the fact that CLEA can be made from heterogeneous populations of proteins/enzymes. Porcine pancreatic acetone powder crude extract was used for preparing CLEA in such a way that lipase, α-amylase, phospholipase A2 activities were retained upto 100%. The lipase present in the CLEA showed greater thermal stability at 50 °C as compared to free enzyme. For lipase and phospholipase A2, Vmax/Km showed no significant change upon combi-CLEA formation but decreased significantly for α-amylase activity from 190 to 114 min−1. The lipase activity and α-amylase activity in CLEA were completely retained upto three cycles of use. The scanning electron microscopic (SEM) studies showed that morphology of CLEA changed upon inactivation by reuses.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 44, Issues 3–4, 1 March 2007, Pages 128–132