کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
71307 | 48909 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inactivation studies on native and silica gel non-homogeneous immobilized chloroperoxidase
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A pH stability analysis was made, in the presence of tert-butyl hydroperoxide, of both immobilized and native chloroperoxidase obtained from Caldariomyces fumago and the inactivation constants (j1) evaluated. The native enzyme displays a uni-exponential decay, whereas for the immobilized enzyme a three exponential equation describes the time dependent enzyme inactivation.For immobilized enzyme, three-exponential equation describes the enzyme time-course inactivation. The obtained inactivation constants (j3 and K3) showed an increase in the stability of a fraction of the immobilized enzyme. This is probably due to a decrease of the affinity of the enzyme for the oxidant and not to a decrease in j3 values.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 38, Issue 2, 15 February 2006, Pages 65–72
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 38, Issue 2, 15 February 2006, Pages 65–72
نویسندگان
Paolo Toti, Antonella Petri, Tiziana Gambicorti, Ahmed M. Osman, Carlo Bauer,