کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7228885 | 1470913 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel electrochemical biosensor for antioxidant evaluation of phloretin based on cell-alginate/Ê-cysteine/gold nanoparticle-modified glassy carbon electrode
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Antioxidant evaluation of bioactive compounds is limited, since many methods lack a real physiological environment that can be used conveniently and intuitively. In this study, a simple, label-free and effective electrochemical biosensor method has been developed to evaluate the antioxidant effect of phloretin (Ph) by 3D cell modification on a glassy carbon electrode (GCE). In response to this, A549 cells were immobilized onto a self-assembled Ê-cysteine/gold nanoparticle (AuNPs/Ê-Cys)-modified GCE surface by a simple drop casting after encapsulated in alginate. The electrochemical impedance spectroscopy (EIS) results showed that the impedance value (Ret) increased with the concentration of H2O2 in the range of 0-60â¯Î¼mol/L with the correlation of 0.990 which acted as an oxidative stress model inducer. However, the EIS value decreased with the co-incubation of Ph ranging from 10 to 100â¯Î¼mol/L, showing a dose-dependent manner and time effect, indicating that the variation of Ret was responded to the antioxidant effect. The response impedance of the biosensor is linear to Ph concentrations from 20â¯Î¼mol/L to 100â¯Î¼mol/L with the detection limit (LOD) as 1.96â¯Î¼mol/L. A significant correlation was observed between reactive oxygen species (ROS) values and Ret values following the concentrations of Ph, thus demonstrating the good biological relevance of cell-based electrochemical method. The strategy has been used to evaluate Ph antioxidant capacity in real cells with satisfactory results, indicating the feasibility of biosensor analysis for antioxidant evaluation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 119, 15 November 2018, Pages 119-125
Journal: Biosensors and Bioelectronics - Volume 119, 15 November 2018, Pages 119-125
نویسندگان
Yongli Ye, Jian Ji, Fuwei Pi, Huicheng Yang, Jie Liu, Yinzhi Zhang, Shuang Xia, Jiasheng Wang, Dan Xu, Xiulan Sun,