کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7228924 | 1470914 | 2018 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free distinction between p53+/+ and p53 -/- colon cancer cells using a graphene based SERS platform
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Surface-Enhanced Raman Scattering (SERS) is used to differentiate two colon cancer cell line HCT 116, that is, to distinguish a TP53 gene knockout cell line (p53 -/-) from a wild type (p53 +/+). A label-free graphene/gold nanopyramid based SERS platform, combined with the multivariate analysis: principal component analysis, is used to profile live, dead, and burst colon cancer cells suspended in simulated body fluid (SBF). The graphene sheet permits SERS hotspot identification and provides a chemical enhancement for the biological constituents. This study found that a unique fingerprint exists for three different states of the cell, burst, live, and dead, which were used to differentiate the p53 +/+ and p53 -/- cell lines. Perceptron with Pocket Algorithm was also coupled with PCA to demonstrate an average of 81% sensitivity and 97% specificity in separating the two cell lines. The demonstration of single gene differentiation shows the great applicable potential of this SERS graphene hybrid platform for cancer diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 118, 30 October 2018, Pages 108-114
Journal: Biosensors and Bioelectronics - Volume 118, 30 October 2018, Pages 108-114
نویسندگان
Owen Liang, Pu Wang, Ming Xia, Catherine Augello, Fan Yang, Gang Niu, Huinan Liu, Ya-Hong Xie,