کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230741 | 1470951 | 2016 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free immunosensor based on one-step electrodeposition of chitosan-gold nanoparticles biocompatible film on Au microelectrode for determination of aflatoxin B1 in maize
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کلمات کلیدی
SPCEMBATHIMSCAflatoxin B1 - آفلاتوکسین B14-Mercaptobenzoic acid - اسید 4-مروپپتوبنزوئیکElectrodeposition - الکترودScreen-printed carbon electrode - الکترود کربن روی صفحه نمایشImmunosensor - ایمونوسنسورThionine - تونینCysteamine - سیستامینIonic liquid - مایع یونیMicroelectrode - میکروالکترودGold nanoparticle - نانو ذرات طلاConducting polymer - پلیمرهای رسانا Mesoporous carbon - کربن مرطوبChitosan - کیتوسان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Gold nanoparticles (AuNPs) embedded in chitosan (CHI) film, well-dispersed and smaller in size (about 10 nm), were fabricated by one-step electrodeposion on Au microelectrode in solution containing chitosan and chloride trihydrate. The nano-structure CHI-AuNPs composite film offers abundant amine groups, good conductivity, excellent biocompatibility and stability for antibody immobilization. The combination of aflatoxin B1 (AFB1) with immobilized antibody introduces a barrier to electron transfer, resulting in current decreasement. The morphologies and characterizations of modified microelectrodes were investigated by scanning electron microscope (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and Fourier transform infrared spectroscopy (FT-IR). The proposed non-enzyme and label-free immunosensor exhibited high sensitive amperometric response to AFB1 concentration in two linear ranges of 0.1 to 1Â ng mLâ1 and 1 to 30Â ng mLâ1, with the detection limit of 0.06Â ng mLâ1 (S/N=3). The immunoassay was also applied for analysis of maize samples spiked with AFB1. Considering the sample extraction procedure, the linear range and limit of detection were assessed to be 1.6-16Â ng mLâ1 and 0.19Â ng mLâ1 respectively. The simple method showed good fabrication controllability and reproducibility for immunosensor design.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 222-229
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 222-229
نویسندگان
Haihua Ma, Jizhou Sun, Yuan Zhang, Chao Bian, Shanhong Xia, Tong Zhen,