کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7558085 | 1491360 | 2015 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioanalytical method for the simultaneous determination of d- and l-serine in human plasma by LC/MS/MS
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کلمات کلیدی
OPALQCMQCo-phthaldialdehydeHQCN-methyl-d-aspartateNMDAMRMPBSLLOQESILC/MS/MS - LC / MS / MSMS/MS - MS / MSinternal standard - استاندارد داخلیBiomarker - بیومارکرEnantiomer separation - جداسازی انانتیومrelative error - خطای مربوطهBioanalytical method - روش BioanalyticalCoefficient of Variation - ضریب تغییرMass spectrometry - طیف سنجی جرمیTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیmatrix factor - عامل ماتریسSurrogate matrix - ماتریس جایگزینlower limit of quantitation - محدودیت پایین کمیتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریmultiple reaction monitoring - نظارت چندگانه چندگانهliquid chromatography - کروماتوگرافی مایعhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراGas chromatography - کروماتوگرافی گازیquality control - کنترل کیفیتelectrospray ionization - یونیزاسیون الکترو اسپری
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
d-Serine is an endogenous modulator of N-methyl-d-aspartate (NMDA) receptors. Plasma concentrations of d-serine and the ratio of d-serine to total serine may be used as clinically-translatable biomarkers in NMDA receptor-related disease. We developed a highly sensitive and specific method using high performance liquid chromatography tandem mass spectrometry (LC/MS/MS) for the simultaneous determination of the d- and l-isomers of serine in human plasma. Since d- and l-serine are endogenous components, phosphate buffered saline was used as the surrogate matrix. d- and l-serine in human plasma and PBS were treated by cationic exchange solid phase extraction. d-Serine (m/z 106.1 > 60.0), l-serine (m/z 106.1 > 60.1) and dl-serine-d3 (m/z 109.1 > 63.0) were detected using a multiple reaction monitoring. The enantiomer separation of d- and l-serine was successfully achieved without any derivatization step using tandemly-arranged and ice-cold CROWNPAK CR-I(+) columns with an isocratic mobile phase comprised of 0.3% trifluoroacetic acid in 10% acetonitrile. The standard curves were linear throughout the calibration range with 0.01-10 μg/mL (d-serine) and 0.1-100 μg/mL (l-serine), respectively. Intra-day and inter-day precision and accuracy of the quality control samples were within relative standard deviations of less than 15%. The endogenous concentrations of d- and l-serine in human plasma were 0.124-0.199 and 7.97-13.1 μg/mL, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 487, 15 October 2015, Pages 38-44
Journal: Analytical Biochemistry - Volume 487, 15 October 2015, Pages 38-44
نویسندگان
Hiroshi Sugimoto, Masaaki Kakehi, Fumihiro Jinno,