کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7564290 | 1491598 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design and Validation of a Microfluidic Chip with Micropillar Arrays for Three-dimensional Cell Culture
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A microfluidic chip with micropillar arrays for three-dimensional cell culture was designed and validated. With the optimized chip, the mixture of cells and extracellular matrix mimics could be steadily injected into the cell culture chamber, the nutrients in the medium from lateral channels could quickly diffuse into the chamber, and the cell metabolites could also timely diffuse out of the chamber. To test the stability of the microenvironment constructed in the chip, neural stem cells were three-dimensionally cultured.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 45, Issue 8, August 2017, Pages 1109-1114
Journal: Chinese Journal of Analytical Chemistry - Volume 45, Issue 8, August 2017, Pages 1109-1114
نویسندگان
Jun-Shan LIU, Yang-Yang ZHANG, Zhong WANG, Jia-Yi DENG, Xuan YE, Ri-Ye XUE, Dan GE, Zheng XU,