کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7586427 | 1492047 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel aspartic protease from Rhizomucor miehei expressed in Pichia pastoris and its application on meat tenderization and preparation of turtle peptides
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کلمات کلیدی
High level expressionHPSECN-cyclohexyl-2-aminoethanesulfonic acidORFRhizomucor mieheiCHESMOPS2-(N-morpholino) ethanesulfonic acid - 2- (N-مورفولینو) اتان سولفونیک اسیدSodium dodecyl sulfatepolyacrylamide gel electrophoresis - الکتروفورز ژل سدیم دودسیل سولفات پلیا اکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدMeat tenderization - تمیز کردن گوشتopen reading frame - قاب خواندن بازMeS - مسpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازProtease - پروتئازPichia pastoris - پیکیا پاستوریسhigh performance size exclusion chromatography - کروماتوگرافی خروج با اندازه کافی بالا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel aspartic protease from Rhizomucor miehei expressed in Pichia pastoris and its application on meat tenderization and preparation of turtle peptides A novel aspartic protease from Rhizomucor miehei expressed in Pichia pastoris and its application on meat tenderization and preparation of turtle peptides](/preview/png/7586427.png)
چکیده انگلیسی
A novel aspartic protease gene (RmproA) was cloned from the thermophilic fungus Rhizomucor miehei CAU432 and expressed in Pichia pastoris. The RmproA was successfully expressed in P. pastoris as an active extracellular protease. High protease activity of 3480.4â¯U/mL was obtained by high cell-density fermentation. The protease was purified by the two step protocols to homogeneity. The molecular mass of the RmproA was estimated to be 52.4â¯kDa by SDS-PAGE and 50.6â¯kDa by gel filtration. The purified enzyme was optimally active at pH 5.5 and 55â¯Â°C, respectively. The enzyme exhibited a broad range of substrate specificity. RmproA-treated pork muscle showed lower shear force than papain-treated sample at a relative low concentration, suggesting its effectiveness on meat tenderization. Moreover, turtle hydrolysis by RmproA resulted in a large amount of small peptides, which exhibited high ACE-inhibitory activity. Thus, RmproA may be a potential candidate for several industrial applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 245, 15 April 2018, Pages 570-577
Journal: Food Chemistry - Volume 245, 15 April 2018, Pages 570-577
نویسندگان
Qian Sun, Fusheng Chen, Fang Geng, Yongkang Luo, Siyi Gong, Zhengqiang Jiang,