کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7586994 | 1492078 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid method for the simultaneous quantification of the major tocopherols, carotenoids, free and esterified sterols in canola (Brassica napus) oil using normal phase liquid chromatography
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کلمات کلیدی
γ-Tocopherol (PubChem CID: 92729)δ-Tocopherol (PubChem CID: 92094)Campesterol (PubChem CID: 173183)β-sitosterol (PubChem CID: 222284)Brassicasterol (PubChem CID: 5281327)Tandem quadrupole mass spectrometryα-Tocopherol (PubChem CID: 14985) - α-توکوفرول (PubChem CID: 14985)β-Carotene (PubChem CID: 5280489) - β-کاروتن (PubChem CID: 5280489)Simultaneous analysis - تجزیه و تحلیل همزمانBioactive compounds - ترکیبات بیولوژیکیEdible oil - روغن خوراکیLutein (PubChem CID: 5281243) - لوتئین (PubChem CID: 5281243)high performance liquid chromatography - کروماتوگرافی مایع با کارایی بالا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A normal phase high performance liquid chromatography (HPLC) method was developed to simultaneously quantify several prominent bioactive compounds in canola oil vis. α-tocopherol, γ-tocopherol, δ-tocopherol, β-carotene, lutein, β-sitosterol, campesterol and brassicasterol. The use of sequential diode array detection (DAD) and tandem mass spectrometry (MS/MS) allowed direct injection of oils, diluted in hexane without derivatisation or saponification, greatly reducing sample preparation time, and permitting the quantification of both free sterols and intact sterol esters. Further advantages over existing methods included increased analytical selectivity, and a chromatographic run time substantially less than other reported normal phase methods. The HPLC-DAD-MS/MS method was applied to freshly extracted canola oil samples as well as commercially available canola, palm fruit, sunflower and olive oils.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 214, 1 January 2017, Pages 147-155
Journal: Food Chemistry - Volume 214, 1 January 2017, Pages 147-155
نویسندگان
Clare L. Flakelar, Paul D. Prenzler, David J. Luckett, Julia A. Howitt, Gregory Doran,