کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7596519 | 1492125 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of an online-SPE-LC-MS method for the investigation of the intestinal absorption of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP) and its bacterial metabolite PHIP-M1 in a Caco-2 Transwell system
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کلمات کلیدی
SPEPFPHCAPhIPFCSTEER2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine - 2-amino-1-methyl-6-phenylimidazo [4،5-b] pyridineAutomated sample preparation - آماده سازی نمونه های خودکارHeterocyclic aromatic amine - آمین های معطر heterocyclicSolid phase extraction - استخراج فاز جامدcollision energy - انرژی برخوردIntestinal absorption - جذب رودهfetal calf serum - سرم گوساله جنینapparent permeability coefficient - ضریب نفوذپذیری ظاهریtransepithelial electrical resistance - مقاومت الکتریکی transepithelialPapp - پاپ
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of an online-SPE-LC-MS method for the investigation of the intestinal absorption of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP) and its bacterial metabolite PHIP-M1 in a Caco-2 Transwell system Development of an online-SPE-LC-MS method for the investigation of the intestinal absorption of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP) and its bacterial metabolite PHIP-M1 in a Caco-2 Transwell system](/preview/png/7596519.png)
چکیده انگلیسی
Heterocyclic aromatic amines such as PHIP are formed during the heat processing of food. PHIP undergoes bacterial metabolism leading to 7-hydroxy-5-methyl-3-phenyl-6,7,8,9-tetrahydropyrido[3â²,2â²:4,5]imidazo[1,2-a]pyrimidin-5-ium chloride (PHIP-M1) as main metabolite. We developed an LC-MS method with automated sample preparation by online-solid-phase-extraction for the simultaneous quantification of PHIP and its mammalian and bacterial metabolites N-hydroxy-PHIP, 4-OH-PHIP and PHIP-M1 in biological samples. The method was used to investigate the transport of PHIP-M1 through a Caco-2 cell monolayer. The experiments show that PHIP-M1 rapidly crosses the cell monolayer and that PHIP-M1 is a substrate for P-glycoprotein and the multiple drug resistance 2 transporter. The intestinal absorption of PHIP-M1 is comparable with that of PHIP and a moderate to high bioavailability has to be expected. Thus, not only the human metabolites of PHIP but also the bacterial metabolite PHIP-M1 formed in the gut could contribute to the toxic effects of PhIP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 166, 1 January 2015, Pages 537-543
Journal: Food Chemistry - Volume 166, 1 January 2015, Pages 537-543
نویسندگان
Ina Willenberg, Leonie von Elsner, Pablo Steinberg, Nils Helge Schebb,