کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7607505 | 1493359 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolomic analysis of mammalian cells and human tissue through one-pot two stage derivatizations using sheathless capillary electrophoresis-electrospray ionization-mass spectrometry
ترجمه فارسی عنوان
تجزیه و تحلیل متابولومیک سلول های پستانداران و بافت انسان از طریق مشتقات دو مرحلهای یک گلدان با استفاده از طیف سنجی جرم یونیزاسیون الکتروفورز الکتروپروسسوری مویرگی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Analysis of metabolites is often performed using separations coupled to mass spectrometry which is challenging due to their vast structural heterogeneity and variable charge states. Metabolites are often separated based on their class/functional group which in large part determine their acidity or basicity. This charge state dictates the ionization mode and efficiency of the molecule. To improve the sensitivity and expand the coverage of the mammalian metabolome, multifunctional derivatization with sheathless CE-ESI-MS was undertaken. In this work, amines, hydroxyls and carboxylates were labeled with tertiary amines tags. This derivatization was performed in under 100â¯min and resulted in high positive charge states for all analytes investigated. Amino acids and organic acids showed average limits of detection of 76â¯nM with good linearity of 0.96 and 10% RSD for peak area. Applying this metabolomic profiling system to bovine aortic endothelial cells showed changes in 15 metabolites after treatment with high glucose. The sample injection volume on-capillary was <300 cells for quantitative analyses. Targeted metabolites were found in human tissue, which indicates possible application of the system complex metabolome quantitation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1567, 14 September 2018, Pages 219-225
Journal: Journal of Chromatography A - Volume 1567, 14 September 2018, Pages 219-225
نویسندگان
Tianjiao Huang, Michael Armbruster, Richard Lee, Dawn S. Hui, James L. Edwards,