کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7608596 | 1493384 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Parallel loading and complete automation of a 3-step mAb purification process for multiple samples using a customized preparative chromatography instrument with networked pumps
ترجمه فارسی عنوان
بارگیری موازی و کامل سازی یک فرآیند تصفیه 3 مرحله ای مؤثر برای چندین نمونه با استفاده از یک ابزار کروماتوگرافی آماده سازی سفارشی با پمپ های شبکه
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Advancement in high-throughput screening methods of novel therapeutic proteins for early stage research and development, specifically mAbs, have given mid-scale (milligram to gram scale) purification groups access to more of these molecules. The available purification technologies built to support mid-scale production was not efficient or versatile enough to keep up with this surge. To remedy this problem, we have designed and built a custom instrument using an ÃKTA Pure. This system enables parallel processing up to 5 samples and has the versatility to perform 1- to 3-step purification processes in a single queue. Furthermore, a unique purification scheme can be selected for each of the five samples in the queue. Overall processing time has reduced by 83% compared to manual, non-parallel load methods. Here, we describe our novel approach and demonstrate the flexibility, speed and efficiency of the instrument.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1542, 23 March 2018, Pages 50-60
Journal: Journal of Chromatography A - Volume 1542, 23 March 2018, Pages 50-60
نویسندگان
Darren E. Ferguson, Evan R. Mahan, Weijun Ma, Gabrielle Bitzas, Xiaotian Zhong, Richard Zollner, Aaron M. D'Antona,