Solid phase extraction based on porous magnetic graphene oxide/β-cyclodextrine composite coupled with high performance liquid chromatography for determination of antiepileptic drugs in plasma samples

In this study, a novel solid phase extraction based on porous magnetic graphene oxide-cyclodextrin polymers (MGO-CD) was developed with aromatic linker and used for the simultaneous extraction and preconcentration of trace antiepileptic drugs in plasma. The characteristics of MGO-CD were assessed by Fourier transform infrared spectroscopy (FT-IR), Brunauer-Emmett-Teller surface areas (BET), Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). Magnetic cyclodextrin polymeric material exhibited a high specific surface area (187 m2 g−1), large pore volume (0.12 cm3 g−1), good saturated magnetization (37.68 emu g−1), and excellent dispersibility in aqueous solution. Various parameters influencing the extraction recovery of drugs were studied. The method exhibited good linearity in the range of 1-50 μg mL−1, and the limit of quantitation of carbamazepine, phenytoin, diazepam was 11.89, 47.10 and 16.17 ng mL−1, respectively. The recoveries of three antiepileptic drugs range from 78.49 to 100.93% with relative standard deviations less than 5.5%. These analytical results demonstrated that the proposed approach based on MGO-CD was applicable for analysis of antiepileptic drugs in plasma samples.