کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7609846 | 1493403 | 2017 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dual-wavelength light-emitting diode-based ultraviolet absorption detector for nano-flow capillary liquid chromatography
ترجمه فارسی عنوان
دو سنجنده جذب نور ماوراء بنفش مبتنی بر دیود نور نوری برای کروماتوگرافی مایع مغناطیسی نانو
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The design of a miniaturized LED-based UV-absorption detector was significantly improved for on-column nanoflow LC. The detector measures approximately 27 mm Ã 24 mm Ã 10 mm and weighs only 30 g. Detection limits down to the nanomolar range and linearity across 3 orders of magnitude were obtained using sodium anthraquinone-2-sulfonate as a test analyte. Using two miniaturized detectors, a dual-detector system was assembled containing 255 nm and 275 nm LEDs with only 216 nL volume between the detectors A 100 μm slit was used for on-column detection with a 150 μm i.d. packed capillary column. Chromatographic separation of a phenol mixture was demonstrated using the dual-detector system, with each detector producing a unique chromatogram. Less than 6% variation in the ratios of absorbances measured at the two wavelengths for specific analytes was obtained across 3 orders of magnitude concentration, which demonstrates the potential of using absorption ratio measurements for target analyte detection. The dual-detector system was used for simple, but accurate, mobile phase flow rate measurement at the exit of the column. With a flow rate range from 200 to 2000 nL/min, less than 3% variation was observed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1523, 10 November 2017, Pages 242-247
Journal: Journal of Chromatography A - Volume 1523, 10 November 2017, Pages 242-247
نویسندگان
Xiaofeng Xie, Luke T. Tolley, Thy X. Truong, H. Dennis Tolley, Paul B. Farnsworth, Milton L. Lee,