کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7610498 | 1493497 | 2016 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Screening and confirmatory method for multiclass determination of 62 antibiotics in meat
ترجمه فارسی عنوان
روش غربالگری و تایید کننده برای تعیین چند قطبی 62 آنتی بیوتیک در گوشت
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کلمات کلیدی
آنتی بیوتیک ها، عضله، کروماتوگرافی مایع با طیف سنجی با وضوح بالا، اعتبار سنجی روش،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A multiclass method for screening and confirmatory analysis of antimicrobial residues in muscle has been developed and validated, according to Commission Decision 2002/657/EC. Sixty-two antibiotics belonging to ten different drug families (amphenicols, beta-lactams, diamino-pyrimidine, lincosamides, macrolides, pleuromutilins, quinolones, rifamycins, sulfonamides and tetracyclines) have been included in the method. After the addition of an aqueous solution of EDTA, the minced muscle was extracted with acetonitrile/water mixture and, later, with pure acetonitrile. The extract was evaporated and redissolved in ammonium acetate buffer prior to LC injection. Instrumental determination was performed by liquid chromatography coupled to hybrid high resolution mass analyser (LC-HRMS/MS) operating in positive electrospray ionization mode. Chromatographic separation was optimized on a Poroshell 120 EC-C18 column (100 Ã 3.0 mm, 2.7 μm) with gradient using methanol and water containing 0.1% of formic acid as mobile phases. The method was validated in bovine muscle in the range 3.3-150 μg kgâ1 for all antibiotics; for some compounds with MRL higher than 100 μg kgâ1, the validation interval has been extended until 1500 μg kgâ1. The studied performance characteristics were selectivity, linearity, precision, trueness (recovery), decision limits, detection capabilities, detection and quantification limits. Satisfactory quantitative performances were obtained for all the analytes. Ruggedness tests demonstrated the applicability to swine and poultry muscle, too. Finally the wide participation in proficiency tests allowed to investigate in deep the method performances.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1429, 15 January 2016, Pages 175-188
Journal: Journal of Chromatography A - Volume 1429, 15 January 2016, Pages 175-188
نویسندگان
Simone Moretti, Guglielmo Dusi, Danilo Giusepponi, Simona Pellicciotti, Rosanna Rossi, Giorgio Saluti, Gabriele Cruciani, Roberta Galarini,