کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7611617 1493518 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two-dimensional high performance liquid chromatography for determination of homocysteine, methionine and cysteine enantiomers in human serum
ترجمه فارسی عنوان
کروماتوگرافی مایع کروماتوگرافی با کارایی دو بعدی برای تعیین آنتی آنومیم های هوموسیستئین، متیونین و سیتئین در سرم انسان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A two-dimensional HPLC system with electrochemical detection was used for determination of homocysteine, methionine and cysteine enantiomers in biological samples. The amino acid separations were not possible only by using a chiral column. The compounds were separated from each other on an achiral column (Purospher RP-18 endcapped 250-4 mm, 5 μm) and their enantiomers were separated on Chirobiotic TAG (250-4.6 mm, 5 μm) column in an on-line system. The mobile phase composition and a choice of electrode potentials for detection were investigated. The l-enantiomers always eluted before the d-enantiomers. The proposed method was applied to the analysis of human serum of healthy volunteers and patients with multiple sclerosis. The limit of detection (LOD) and quantitation (LOQ) were defined as the concentration that produced a signal-to-noise ratio (S/N) of 3 and 10. The method LOD values were found to be between 0.05 and 0.50 μg mL−1. The range of LOQ values were between 0.17 and 1.67 μg mL−1, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1408, 21 August 2015, Pages 118-124
نویسندگان
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