Determination of phosphatidylserine in milk-based nutritional products using online derivatization high-performance liquid chromatography

Phosphatidylserine (PS) has received interest for its ability to improve cognitive abilities and behaviors. A new method for determining PS in milk-based nutritional products has been developed. The method includes a quick and simple sample preparation procedure, followed by high-performance liquid chromatography (HPLC) fluorescence detection (FLD) with an on-line 9-fluorenylmethyloxycarbonyl (FMOC) derivatization. The method allows PS to be determined in raw materials, milk powder and liquid milk products. The day-to-day (n = 3 days) average recovery of over spike-in (at 100% PS content level) was 100%, and the method quantification limit is 53 mg per kg milk powder.