کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7613383 1493588 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction of arginine with Capto MMC in multimodal chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Interaction of arginine with Capto MMC in multimodal chromatography
چکیده انگلیسی
This study highlights the ability of arginine to elute bovine serum albumin (BSA) and a monoclonal antibody against interleukin-8 (mAb-IL8) from Capto MMC, which is a multimodal cation exchanger. Arginine provides high recovery of monomeric BSA from Capto MMC chromatography columns at yields similar to NaCl elution, and oligomeric BSA was more readily eluted by arginine than by NaCl. The effectiveness of arginine as an eluent also enabled the separation of monomeric BSA from the oligomeric forms. The purification of mAb-IL8 was successfully achieved using Capto MMC chromatography and arginine as the eluent. The mechanism of the effects of arginine on protein elution was determined by calculating the binding free energy between arginine and Capto MMC using molecular dynamics simulations. The overall affinity of arginine for Capto MMC was associated with electrostatic interactions. However, additional affinities contributed by hydrophobic interaction or hydrogen bonding were also observed to play a role in the interaction between arginine and Capto MMC, which likely results in the characteristic elution by arginine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1338, 18 April 2014, Pages 58-66
نویسندگان
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