Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection

► A novel capillary electrophoresis noncompetitive immunoassay was proposed. ► The assay was based on AuNPs amplified CL detection to enhance sensitivity. ► This protocol provides a new method for the detection of carcinoembryonic antigen. ► The method showed high sensitivity, selectivity and good repeatability.