کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614800 | 1493976 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Semi-quantitative profiling of bile acids in serum and liver reveals the dosage-related effects of dexamethasone on bile acid metabolism in mice
ترجمه فارسی عنوان
نمایه سازی نیمه کمی اسیدهای صفراوی در سرم و کبد، اثرات مرتبط با دوز دگزامتازون بر متابولیسم اسید های صفراوی را در موش نشان می دهد
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کلمات کلیدی
MCAunconjugated bile acidsSecondary bile acidsPXRCDCAUPLCLC-MSDEXChenodeoxycholic acid - اسید ChenodeoxycholicBile acid - اسید صفراویmuricholic acid - اسید چربCholic acid - اسید چلیکBile acids - اسیدهای صفراویtotal bile acids - اسیدهای صفراوی کلDexamethasone - دگزامتازونUltra-performance liquid chromatography - کروماتوگرافی مایع فوق العاده عملکردیPregnane X receptor - گیرنده پیش گران Xglucocorticoid receptor - گیرنده گلوکوکورتیکوئید
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Nuclear receptors, such as the pregnane X receptor (PXR) and glucocorticoid receptor (GR), play an important role in regulating the homeostasis of bile acids (BAs). In previous studies, two-week treatment of 1â¯mg/kg of dexamethasone (DEX) was used to activate GR in mice, whereas 4-day treatment of 75â¯mg/kg of DEX was chosen to activate PXR. However, little is known about the effect of DEX on circulating and hepatic BA profiles. In the present study, we reported a simple and rapid LC-MS method for semi-quantitative profiling of 39 BA species in mouse serum and liver. This method was applied to investigate the BA profiles in mice treated with either 1â¯mg/kg DEX for two weeks or 75â¯mg/kg DEX for 4â¯days. The gene expression, microsomal induction and liver enlargement in mice confirmed that PXR was activated by 4-day treatment of 75â¯mg/kg DEX, but not by two-week treatment of 1â¯mg/kg DEX. Two-week treatment of 1â¯mg/kg DEX markedly increased the circulating BAs, in particular conjugated primary BAs, suggesting a pro-cholestatic effect of DEX at low doses. In contrast, 4-day treatment of 75â¯mg/kg DEX increased BA hydroxylation and decreased hepatic BAs, in particular unconjugated secondary BAs, suggesting a BA-lowering and bacteria-suppressive effect of DEX at high doses. To conclude, a semi-quantitative LC-MS method was developed and applied to elucidate the dosage-related effects of DEX on serum and hepatic BA profiles in mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1095, 15 September 2018, Pages 65-74
Journal: Journal of Chromatography B - Volume 1095, 15 September 2018, Pages 65-74
نویسندگان
Xue Wang, Fangyu Wang, Zhiqiang Lu, Xinghua Jin, Youcai Zhang,