کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614985 | 1493978 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous quantification of antibiotics in wastewater from pig farms by capillary electrophoresis
ترجمه فارسی عنوان
اندازه گیری همزمان آنتی بیوتیک ها در فاضلاب از مزارع خوک توسط الکتروفورز مویرگی
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کلمات کلیدی
الکتروفورز مویرگی، آنتی بیوتیک ها، فلوروکینولون ها، تتراسیکلین ها، فاضلاب،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Pig farming is an important activity in the economic development of Mexico with millions of tons of meat produced annually. Antibiotics are used in therapeutic dose to prevent diseases, and sometimes as growth promoters. These compounds are not completely metabolized; they are carried into the environment in its active form at concentrations that could induce antibiotic resistance in bacteria, which could be transferred to human pathogens by horizontal gene transfer. The objective of this work was to develop methods of analysis for simultaneous quantification of the antibiotics Oxytetracycline (OXT), Chlortetracycline (CLT), Enrofloxacin (ENRO) and Ciprofloxacin (CIPRO) by field-amplified sampling injection in capillary zone electrophoresis (FASI-CZE). The method was validated by parameters of (1) linearity, obtaining a lineal range of 0.05 at 1â¯Î¼gâ¯mLâ1 for ENRO and CIPRO, and from 0.1 to 1â¯Î¼gâ¯mLâ1 for OXT and CLT; (2) precision, obtaining values <5% of standard deviation for CIPRO and ENRO and <10% of standard deviation for OXT and CLT; (3) accuracy, with recovery values from 93 to 115%; (4) selectivity, with values of resolution >2 for the all antibiotics tested. To prove the method, a sample of wastewater from a local pig farm was analyzed, detecting a concentration of 0.140â¯Â±â¯0.009 for OXT. This concentration was higher than the minimal selective concentration, indicating the point in which resistance to a determined antibiotic could develop. The methods were validated with precision and sensitivity comparable to chromatographic methods, which can be used to analyze wastewater from pig farms directly.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 386-393
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 386-393
نویسندگان
Carlos A. DÃaz-Quiroz, J. Francisco Hernández-Chávez, Gabriela Ulloa-Mercado, Pablo Gortáres-Moroyoqui, Rosario MartÃnez-MacÃas, Edna Meza-Escalante, Denisse Serrano-Palacios,