کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615066 | 1493983 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid determination of 3â², 4â²-dimethoxy flavonol-3-β-d-glucopyranoside in rat plasma by LC-MS/MS method followed by protein precipitation
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کلمات کلیدی
LQCMQCLLOQESIHQCMRMRSDLC-MS/MS - LC-MS / MSinternal standard - استاندارد داخلیrelative standard deviation - انحراف استاندارد نسبیstandard deviation - انحراف معیارlower limit of quantification - حد پایین کمیت سنجیrelative error - خطای مربوطه96-Well format - فرمت 96 اینچیFlavonoids - فلاونوئیدهاPharmacokinetic study - مطالعه فارماکوکینتیکmultiple reaction monitoring - نظارت چندگانه چندگانهLiquid chromatography/tandem mass spectrometry - کروماتوگرافی مایع / طیف سنج جرم دو طرفهhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراhigh quality control - کنترل با کیفیت بالاquality control - کنترل کیفیت
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A reliable liquid chromatography/tandem mass (LC-MS/MS) method was developed for quantitation of 3â², 4â²-dimethoxy flavonol-3-β-d-glucopyranoside (DF3G) in rat plasma using pantoprazole as the internal standard. An Agilent C18 column (100â¯mmâ¯Ãâ¯3â¯mm, 3.5â¯Î¼m) was performed for chromatographic separation with the mobile phase composed of methanol-water (containing formic acid) at a flow rate of 0.4â¯mL/min. A triple-quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source in positive ion mode was applied to quantitative analysis by multiple reacting monitoring (MRM). The MRM precursor-product ion transition of DF3G and Pantoprazole (IS) were m/z 461.1â¯ââ¯299.2 and 383.9â¯ââ¯200.2, respectively. Calibration curves were recovered in a concentration range of 1-500â¯ng/mL for plasma with a limit of lower quantification (LLOQ) of 1â¯ng/mL. The intra-day and inter-day precision were not >10%. The accuracy of DF3G ranged from â10% to 0.53% in quality control (QC) samples at three concentrations. DF3G was not degraded during the analysis and the storage period. All the data were validated in accordance with the FDA bioanalytical method validation guideline. The LC-MS/MS method was successfully employed in the pharmacokinetic study of the DF3G after oral administration and intravenous injection in rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1086, 1 June 2018, Pages 47-55
Journal: Journal of Chromatography B - Volume 1086, 1 June 2018, Pages 47-55
نویسندگان
Qiong Lou, Jun Wen, Yuanying Jiang, Jinghua Huang, Guorong Fan, Fenyun Song,