کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7616467 | 1494040 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An optimized method for neurotransmitters and their metabolites analysis in mouse hypothalamus by high performance liquid chromatography-Q Exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry
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کلمات کلیدی
5-HTDNS-Cll-AA3-methoxy-4-hydroxyphenylglycolMHPG5-hydroxytryptophanNTS5-HIAADOPACQ-TOF5-hydroxytryptamineMRM5-HTPLOQSRM3,4-dihydroxyphenylacetic acid - 3،4-دی هیدروکسی فنیل اسیدهای اسید5-hydroxyindole-3-acetic acid - 5-hydroxyindole-3-acetate اسیدl-Tyrosine - L-TyrosineLC–MS/MS - LC-MS / MSinternal standard - استاندارد داخلیγ-aminobutyric acid - اسید γ-آمینوبوتیریکNeurotransmitters - انتقالدهندههای عصبی Epinephrine - اپی نفرینQuantitative detection - تشخیص کمیLOD یا Limit of detection - حد تشخیصDansyl chloride - دانسیل کلرایدDopamine - دوپامینquadrupole time-of-flight - زمان پرواز چهار روزهCoefficient of Variation - ضریب تغییرMass spectrometry - طیف سنجی جرمیlimit of quantification - محدودیت اندازه گیریlimit of detection - محدودیت تشخیصMouse - موشselected reaction monitoring - نظارت بر واکنش انتخاب شدهmultiple reaction monitoring - نظارت چندگانه چندگانهnorepinephrine - نوراپی نفرینhomovanillic acid - هومووانیلیک اسیدHypothalamus - هیپوتالاموسHVA - چهCaffeic acid - کافئیک اسیدliquid chromatography coupled with tandem mass spectrometry - کروماتوگرافی مایع همراه با طیف سنجی جرمی دو طرفهHPLC - کروماتوگرافی مایعی کاراhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراquality control - کنترل کیفیتGABA - گابا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Neurotransmitters (NTs) and their metabolites are known to play an essential role in maintaining various physiological functions in nervous system. However, there are many difficulties in the detection of NTs together with their metabolites in biological samples. A new method for NTs and their metabolites detection by high performance liquid chromatography coupled with Q Exactive hybrid quadruple-orbitrap high-resolution accurate mass spectrometry (HPLC-HRMS) was established in this paper. This method was a great development of the applying of Q Exactive MS in the quantitative analysis. This method enabled a rapid quantification of ten compounds within 18Â min. Good linearity was obtained with a correlation coefficient above 0.99. The concentration range of the limit of detection (LOD) and the limit of quantitation (LOQ) level were 0.0008-0.05Â nmol/mL and 0.002-25.0Â nmol/mL respectively. Precisions (relative standard deviation, RSD) of this method were at 0.36-12.70%. Recovery ranges were between 81.83% and 118.04%. Concentrations of these compounds in mouse hypothalamus were detected by Q Exactive LC-MS technology with this method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1012â1013, 15 February 2016, Pages 79-88
Journal: Journal of Chromatography B - Volumes 1012â1013, 15 February 2016, Pages 79-88
نویسندگان
Zong-Lin Yang, Hui Li, Bing Wang, Shu-Ying Liu,