کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617892 | 1494083 | 2014 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid screening and identification of α-amylase inhibitors from Garcinia xanthochymus using enzyme-immobilized magnetic nanoparticles coupled with HPLC and MS
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کلمات کلیدی
EFRBFRPFRFITCnuclear magnetic resonance - رزونانس مغناطیسی هستهای3-Aminopropyltrimethoxysilane - 3-آمینوپروپیل تریمتوکسیسیلانDMSO - DMSOMNPs - MNP هاNMR - تشدید مغناطیسی هستهای Dimethyl sulfoxide - دیمتیل سولفواکسیدmass spectra - طیف جرمیFT-IR - طیف سنجی مادون قرمز تبدیل فوریهFourier transform infrared spectroscopy - طیف سنجی مادون قرمز تبدیل فوریه یا طیف سنجی FTIRfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتAPTES - مناسبFe3O4 magnetic nanoparticles - نانوذرات مغناطیسی Fe3O4high performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراGlutaraldehyde - گلوتارالدئید
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
α-Amylase inhibitors play an important role in management of diabetes and obesity. In order to rapidly discover potent α-amylase inhibitors from medicinal plants, a ligands-screening method based on enzyme-immobilized magnetic nanoparticles integrated with HPLC was developed. Amine-terminated magnetic nanoparticles were prepared for the immobilization of α-amylase. Based on the affinity theory, the α-amylase-coated magnetic nanoparticles were employed to fish out the ligands from the extracts of Garcinia xanthochymus, and the elutes were examined by HPLC. As a result, three ligands were screened out. Isolation and identification were carried out subsequently. By analyzing the UV, MS and NMR spectra, they were identified as three biflavonoids including GB2a glucoside (2), GB2a (3) and fukugetin (4). The IC50 values of the three compounds were also determined. The results suggest the proposed approach is efficient and accurate, and has great potential in rapid discovery of drug candidates from medical plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 960, 1 June 2014, Pages 166-173
Journal: Journal of Chromatography B - Volume 960, 1 June 2014, Pages 166-173
نویسندگان
Yunfang Li, Yu Chen, Chuying Xiao, Dan Chen, Yuxiu Xiao, Zhinan Mei,