کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625732 | 1494575 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of free thyroxine determination based on one-step fluorometric immunoassay technique and the suboptimal concordance with two-step immunoassay
ترجمه فارسی عنوان
بررسی تیروکسین آزمایش آزاد براساس تکنیک ایمونولوژیک فلورومتریک یک مرحله ای و سازگاری با روش غیرمتمرکز با روش ایمنو دو مرحله ای
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کلمات کلیدی
هورمونهای تیروئید، ایمونوآسیا، ارزیابی تحلیلی، استاندارد سازی، مقایسه روش، اختلافات تکنیک ها،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Free thyroxine (FT4) quantification is continuing to be a concern. The purpose of the following study was to evaluate the analytical performance of Tosoh AIA900 based on a one-step technique and its comparison to Access 2 (two-step technique) over different clinical contexts (euthyroid, thyroid disorders, uncontrolled diabetes, renal failure and pregnancy). The protocol established by the French society of Clinical Biology was used to evaluate: imprecision, limit of detection, trueness, linearity, interferences and method comparisons. Within-run variation of 3.1%, 5.7% and 4.4% were found for the low, medium and high controls, respectively. Between-run was 5.8% for low control, 5.7% for medium control and 7.1% for high control. Common interferences did not affect one-step immunoassay FT4 results. The linearity was checked up to 86â¯pmol/L. The limit of detection was 5.5â¯pmol/L. The concordance correlation coefficient (CCC) showed a low agreement (0.6) between both methods. Bland-Altman plot revealed that AIA 900 one-step immunoassay technique provides a significant higher values ((+2.8â¯Â±â¯2.7â¯pmol/L;pâ¯<â¯0.0001). The Passing-Bablok regression demonstrated both proportional and systematic differences in comparison to Access 2. The lowest association was noted in subjects with impaired renal function (CCCâ¯=â¯0.27). At the time of the study, the results of on-step immunoassay are not directly comparable with Access 2.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 160, 25 October 2018, Pages 187-194
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 160, 25 October 2018, Pages 187-194
نویسندگان
Radhouene Doggui, Rahma Mahjoub, Wassef Lotfi Aissa, Imen Tounsi, Zeineb Allagui, Zmorda Cherif, Sihem Haj Sassi, Sinda Ennigrou, Dhiaeddine Edriss, Jalila El Ati, Amina Bibi,