کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7626418 | 1494579 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An LC-MS/MS method for simultaneous determination of curcumin, curcumin glucuronide and curcumin sulfate in a phase II clinical trial
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Curcumin, a principal curcuminoid of turmeric, gained a lot of attention recently due to its wide spectrum of pharmacological activities in prevention and treatment of various human conditions. The current clinical study is focused on the determination of efficacy and tolerability of curcumin and vitamin D3 combination in patients with early-stage chronic lymphocytic leukemia (CLL) or small lymphocytic lymphoma (SLL). To support this work, an LC-MS/MS method has been developed for simultaneous determination of curcumin and its metabolites, curcumin glucuronide (COG) and curcumin sulfate (COS), in human plasma using curcumin-d6 as an internal standard for curcumin and BPAG-d6 as an internal standard for COG and COS. In this method, plasma samples were prepared by methanol protein precipitation, and the analytes were separated on a Waters XTerra® MS C18 column (2.1â¯mmâ¯Ãâ¯50â¯mm, 3.5â¯Î¼m) using gradient elution with methanol and 10.0â¯mM ammonium formate (pH 3.0) at a flow rate of 0.250â¯mLâ¯minâ1. Quantitation of curcumin, COG, and COS was carried out by tandem mass spectrometry using negative electrospray ionization in multiple-reaction-monitoring (MRM) mode. The linear calibration range for the method was 2.50-500â¯ngâ¯mLâ1 for curcumin, COG, and COS. The method has been validated in accordance with the US-FDA guidelines for bioanalytical method validation. The developed method has been used for the measurement of curcumin, COG and COS concentrations in human plasma samples from a phase II clinical trial.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 156, 15 July 2018, Pages 189-198
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 156, 15 July 2018, Pages 189-198
نویسندگان
Sandeep R. Kunati, Shuming Yang, Basem M. William, Yan Xu,