کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7626923 | 1494583 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of trimethylamine N-oxide, choline, betaine by UPLC-MS/MS in human plasma: An application in acute stroke patients
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Simultaneous determination of trimethylamine N-oxide, choline, betaine by UPLC-MS/MS in human plasma: An application in acute stroke patients Simultaneous determination of trimethylamine N-oxide, choline, betaine by UPLC-MS/MS in human plasma: An application in acute stroke patients](/preview/png/7626923.png)
چکیده انگلیسی
Trimethylamine-N-oxide (TMAO) is derived from the gut microbiome and tissues metabolism of dietary choline and betaine. These molecules are closely related to the development of cardiovascular and cerebrovascular diseases. A rapid, sensitive and accurate method has been developed and validated for the simultaneous determination of trimethylamine N-oxide (TMAO), choline and betaine in human plasma using d9-trimethylamine N-oxide (TMAO), d9-choline, d9-betaine as the internal standard (IS). After methanol precipitation with 10â¯Î¼L plasma samples, the analytes were extracted and then separated on Amide column (2.1â¯Ãâ¯100â¯mm, 1.7â¯Î¼m, waters) with an isocratic elution program consisting of acetonitrile-water (containing 10â¯mM ammonium formate pHâ¯=â¯3.0) at a flow of 400â¯Î¼L/min. The detection was achieved under the selected reaction monitoring (SRM) scan using positive electrospray ionization (ESI+) in 3â¯min. The mass transitions monitored were as follows: m/z 76.3â¯ââ¯58.4 for TMAO, m/z 104.2â¯ââ¯60.3 for choline, m/z 118.1â¯ââ¯58.3 for betaine, m/z 85.1â¯ââ¯66.3 for d9-TMAO, m/z 113.2â¯ââ¯69.3 for d9-choline, and m/z 127.1â¯ââ¯67.2 for d9-betaine, respectively. The method has been fully validated for specificity, lower limit of quantification, linearity, stability, intra- and inter-day accuracy and precision. This assay combines simple sample processing with a short run time and small plasma volumes, making it well suited for high-throughput routine clinical or research purposes. The newly developed method was successfully applied to the patients (nâ¯=â¯220) suffered from acute stroke, and the concentration of choline was firstly found to be closely related with the prognosis of these patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 152, 15 April 2018, Pages 179-187
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 152, 15 April 2018, Pages 179-187
نویسندگان
Weibang Yu, Chuncao Xu, Guomin Li, Weipeng Hong, Ziyi Zhou, Canxing Xiao, Yuanqi Zhao, Yefeng Cai, Min Huang, Jing Jin,