Development and validation of a simple UHPLC-MS/MS method for the simultaneous determination of trimethylamine N-oxide, choline, and betaine in human plasma and urine

- An UHPLC-MS/MS method for measurement of TMAO, choline and betaine in human plasma and urine.
- The method is validated with high accuracy and precision.
- The method is simple with excellent recovery and short run-time of 5 min.
- The method was applied to a patient with kidney disease who underwent kidney transplantation.