Chaotropic salts in liquid chromatographic method development for the determination of pramipexole and its impurities following quality-by-design principles

- LC method with chaotropic additives is developed following the QbD principles.
- Different analytes susceptibility to chaotropic salts effects influenced separation selectivity.
- D-optimal design enabled simultaneous examination of categorical and numerical factors.
- Two ways for the achievement of quality assurance are performed and compared.
- Propagation of error originating from the model coefficients' calculation is more strict approach.