HPLC-DAD and HPLC-ESI-MS analyses of Tiliae flos and its preparations

- An HPLC-DAD-MS method was developed and for the quality control of Tiliae flos preparations.
- 31 constituents were detected and identified.
- Procyanidins were characterised as epicatechin derivatives by 2D-NMR analyses.
- The HPLC method was validated and complied with ICH guidelines.