کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7632694 | 1494663 | 2012 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An indirect sandwich ELISA for the determination of agkisacutacin in human serum: Application to pharmacokinetic study in Chinese healthy volunteers
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کلمات کلیدی
C-type lectin-like proteinPBSFACSACSSmAbLLOQCLPPAbsvWFHRPAes - AESMonoclonal antibody - آنتی بادی مونوکلونالPolyclonal antibody - آنتی بادی های پلی کلونالELISA - تست الیزاLOD یا Limit of detection - حد تشخیصlower limit of quantification - حد پایین کمیت سنجیacute coronary syndromes - سندرم های کرونری حادCoefficient of Variation - ضریب تغییرVon Willebrand factor - عامل فون ویلبراندAdverse events - عوارض جانبیPharmacokinetics - فارماکوکینتیکfluorescence activated cell sorting - فلورسانس سلول فعال فعال سلولlimit of detection - محدودیت تشخیصPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHorseradish peroxidase - پراکسیداز هوررادیشoptical density - چگالی نوری
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The platelet receptor glycoprotein Ib-IX-V complex (GPIb-IX-V) plays a dominant role in the first step of platelet adhesion and arterial thrombus formation. Agkisacutacin, a C-type lectin-like protein (CLP) from Agkistrodon acutus venom, had been previously identified as an antagonist of platelet aggregation and a membrane glycoprotein Ib-binding protein (GPIb-bp). For the analysis of pharmacokinetics of agkisacutacin, an indirect sandwich enzyme-linked immunosorbent assay (ELISA) was established and validated to quantify agkisacutacin in human serum. The method was precise and accurate over the entire linear range of 1.0 and 1000 pg/mL with a lower limit of quantification of 1.0 pg/mL. The intra- and inter-assay coefficient of variation ranged from 0.7 to 4.2% and 1.1 to 4.1%, respectively. Recovery obtained from the accuracy test, using three concentration levels, varied between 96.1 and 110.6%, confirming the assay's reliability. The long-term study showed agkisacutacin was stable at â70 °C up to 46 days. This ELISA was first used to assess the pharmacokinetics of agkisacutacin in healthy volunteers. The characteristics of pharmacokinetic showed that agkisacutacin could rapidly combine with GPIb and slowly dissociate from GPIb-bound form in the body.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 70, November 2012, Pages 396-400
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 70, November 2012, Pages 396-400
نویسندگان
Ya-Nan Zhao, Xiang-Rong Dai, Juan-Juan Liu, Xiang-Hong Li, Jing-Jing Yang, Hua Sun, Ping Wu, Jie Shen, Jian-Ping Lu, Hai-Tang Xie, Xiao-Quan Liu,