Coomassie blue staining for high sensitivity gel-based proteomics

► We report an optimised CBB protocol to enhance quantitative top-down proteomics. ► The selectivity and sensitivity of detection for Coomassie were enhanced. ► The linear dynamic range of the optimised Coomassie was superior to SYPRO Ruby. ► 2DE analyses of mammalian and plant proteomes also reveal superior spot detection.