Principe et applications de la microdissection laser en histopathologie

Normal tissues are composed of different types of cells leading to complex heterogeneous areas. Under the control of a pathologist, laser microdissection is a powerful tool for procuring near-pure populations of targeted cell types from spécifie microscopic regions, or even from a single cell of interest of any tissue sections. This technique, whose main applications will be shown there, is thus able to selectively compare stromal cells and tumor cells in a cancer disease. Within the benefit of new advances in molecular technologies, it has allowed more precise and reproducible analyses during the past decade and has brought new insights to the understanding of a crucial role of DNA, RNA and proteins rather now recognized as biological markers in the tumor area investigations.