کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7661675 | 1495120 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Détermination du 5-méthyltetrahydrofolate dans le sang total par chromatographie liquide
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Détermination du 5-méthyltetrahydrofolate dans le sang total par chromatographie liquide Détermination du 5-méthyltetrahydrofolate dans le sang total par chromatographie liquide](/preview/png/7661675.png)
چکیده انگلیسی
Given the clinical importance of vitamin B9 and its role in many disorders, its determination and particularly of one of its predominant circulating form: 5-methyltetrahydrofolate is a necessity. The present study describes a liquid chromatography method that allows determination of 5-methyltetrahydrofolate in whole blood. Defrosted whole blood samples were deproteinized in an ascorbic acid solution at + 37°C, then submitted to a liquid-liquid partition extraction. The extract is then analyzed by reversed phase liquid chromatography followed by fluorimetric detection within 9 minutes. Different system suitability parameters and validation procedure tests were applied to evaluate the reliability o the proposed method. System suitability parameters were satisfactory for the intended application. Precision results for 5-methyltetrahydrofolate were better than 2 % for chromatographic analysis and better than 5 % for the entire procedure and interassay. Average recovery results was 92.50 %. Limits of detection and quantification were respectively 8 and 10 nmol/l. The proposed liquid chromatography method determines 5-methyltetrahydrofolate in whole blood with a satisfactory reliability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Revue Francophone des Laboratoires - Volume 2008, Issue 407, December 2008, Pages 103-107
Journal: Revue Francophone des Laboratoires - Volume 2008, Issue 407, December 2008, Pages 103-107
نویسندگان
Michèle Aké, Désiré Goin Bi Traye, Christophe Amin N'Cho, François Nicaise Bony, Gildas Komenan Gbassi, Anglade Kla Malan,