کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7682291 | 1495819 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method
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کلمات کلیدی
HRPECR4-MorpholinopyridineBSA - BSAbovine serum albumin - آلبومین سرم گاوEnhancement - ارتقاءOkadaic acid - اسید اوکادائیکELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاShellfish - حلزون صدفدارMorph - مورفChemiluminescence - نورتابی شیمیایی یا کمی لومی نسانسHorseradish peroxidase - پراکسیداز هوررادیش
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method Determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method](/preview/png/7682291.png)
چکیده انگلیسی
A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) was developed to determine okadaic acid (OA). Concentrations of the capture monoclonal anti-OA antibodies, conjugate of OA-HRP and a composition of blocking buffers were varied to optimize the assay condition. The values of IC10, IC50 and working range (IC20-IC80) for CL-ELISA were 0.01, 0.07, and 0.03-0.2Â ng/mL, respectively. Additionally, the analytical recovery values of CL-ELISA from 3 shellfish spiked samples with OA concentrations of 0.03, 0.1 and 0.2Â ng/mL ranged from 86.7% to 111.2%. Closely examining the OA concentrations in 19 various shellfish products performed by CL-ELISA revealed that OA concentrations in 6 of the 19 examined samples was undetected, whereas the 13 samples were contaminated with low levels of OA ranging from 1.2 to 8.0Â ng/g.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 116, 15 November 2013, Pages 343-346
Journal: Talanta - Volume 116, 15 November 2013, Pages 343-346
نویسندگان
Marina M. Vdovenko, Chun-Tse Hung, Ivan Yu. Sakharov, Feng-Yih Yu,