One-step fabrication of integrated disposable biosensor based on ADH/NAD+/meldola's blue/graphitized mesoporous carbons/chitosan nanobiocomposite for ethanol detection

► A rapid and disposable biosensor to detect ethanol was reported. ► The integrated biosensor could be quickly fabricated via one-step drop-coating. ► Graphitized mesoporous carbons (GMCs) were used to adsorb mediator meldola's blue (MDB). ► The prepared biosensor showed the excellent long-term stability. ► The prepared ethanol biosensor was successfully applied to the analysis of real blood samples.