A simple HPLC-ESI-MS method for the direct determination of ten pteridinic biomarkers in human urine

► A new HPLC-ESI-MS method is presented to determine ten pteridinic markers in urine. ► Pteridines are determined in the oxidation level they are excreted. ► Sample preparation was minimized by a direct 10-fold dilution of the urine. ► Pteridine/CREA and NEO/BIO ratios were calculated in urine of healthy volunteers.