Development and validation of an enzyme-linked immunosorbent assay for the quantification of cytochrome 3A4 in human liver microsomes

► An indirect ELISA for the quantification of CYP3A4 in human liver microsomes. ► Polyclonal antibody against full length human CYP3A4 as detection antibody. ► Quantification range of 2 to 300 pmol CYP3A4/mg microsomal protein. ► Successful validation for sensitivity, working range, calibration, accuracy and precision.