Simultaneous measurement of protein-bound 3-chlorotyrosine and homocitrulline by LC-MS/MS after hydrolysis assisted by microwave: Application to the study of myeloperoxidase activity during hemodialysis

► Simultaneous detection of protein-bound 3-chlorotyrosine and homocitrulline. ► Rapid protein acid hydrolysis assisted by microwave oven. ► Method applied to a clinical situation: patients undergoing hemodialysis. ► Myeloperoxidase activity increases during hemodialysis. ► 3-chlorotyrosine but not homocitrulline increases during hemodialysis.