Chemiluminescence determination of timolol maleate by gold nanoparticles-catalyzed luminol-N-bromosuccinimide system

► A new flow injection CL method was developed for timolol maleate. ► AuNPs with the sizes in the range of 6-99 nm was found to catalyze the CL reaction of luminol with N-bromosuccinimide. ► The CL signal was furtherly increased by timolol maleate significantly. ► A thorough discussion on CL reaction mechanism was presented.