Determination of ribavirin in human serum using liquid chromatography tandem mass spectrometry

► Therapeutic drug monitoring of ribavirin is useful to maximize the achievement of sustained virologic response. ► Ultra filtration as a means of sample preparation resulted in the absence of phospholipids in the ultra filtrate and allowed faster chromatography without disturbing matrix. ► This method is suitable for the determination of ribavirin in human serum for therapeutic drug monitoring.