Screening of 4-androstenedione misuse in cattle by LC-MS/MS profiling of glucuronide and sulfate steroids in urine

► LC-MS/MS precursor ion scan mode acquisition was used to detect phase II metabolites. ► 26 phase II metabolites were highlighted as robust biomarkers of 4-AED abuse. ► Statistical PCA model was built to screen 4-AED administration in cattle.